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Covalent immobilization of recombinant fusion proteins with hAGT for single molecule force spectroscopy

✍ Scribed by Stefan K. Kufer; Hendrik Dietz; Christian Albrecht; Kerstin Blank; Angelika Kardinal; Matthias Rief; Hermann E. Gaub


Book ID
105946126
Publisher
Springer
Year
2005
Tongue
English
Weight
498 KB
Volume
35
Category
Article
ISSN
1432-1017

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## Abstract The investigation of native nuclear pore complexes (NPCs) with single‐molecule force spectroscopy requires stable and non‐destructive attachment of the nuclear envelope. Modified, micro‐structured PDMS turns out to be perfectly suited for membrane preparation as it preserves structure a