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Stable, Non-Destructive Immobilization of Native Nuclear Membranes to Micro-Structured PDMS for Single-Molecule Force Spectroscopy

✍ Scribed by Martina Rangl; Reinat Nevo; Ivan Liashkovich; Victor Shahin; Ziv Reich; Andreas Ebner; Peter Hinterdorfer


Book ID
102124423
Publisher
John Wiley and Sons
Year
2009
Tongue
English
Weight
462 KB
Volume
10
Category
Article
ISSN
1439-4235

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Inside Cover: Stable, Non-Destructive Im
✍ Martina Rangl; Reinat Nevo; Ivan Liashkovich; Victor Shahin; Ziv Reich; Andreas 📂 Article 📅 2009 🏛 John Wiley and Sons 🌐 English ⚖ 390 KB

## Abstract The investigation of native nuclear pore complexes (NPCs) with single‐molecule force spectroscopy requires stable and non‐destructive attachment of the nuclear envelope. Modified, micro‐structured PDMS turns out to be perfectly suited for membrane preparation as it preserves structure a