✦ LIBER ✦

Coupling capillary zone electrophoresis and continuous- flow fast atom bombardment mass spectrometry for the analysis of peptide mixtures

✍ Scribed by Richard M. Caprioli; William T. Moore; Millie Martin; Beverly B. DaGue; Kenneth Wilson; Steven Moring

Publisher: Elsevier Science
Year: 1989
Tongue: English
Weight: 638 KB
Volume: 480
Category: Article
ISSN: 1873-3778
DOI: 10.1016/s0021-9673(01)84292-8

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✦ Synopsis

Combined

capillary zone electrophoresis (CZE)-continuous-flow fast atom bombardment (CF-FAB) mass spectrometry is described for the analysis of mixtures of peptides. A 90 cm x 50 pm I.D. fused-silica capillary column was used for electrophoretic separations and was connected to the CF-FAB probe via an interface which allows a total flow into the mass spectrometer of about 5 &min. Solutions of peptides were pneumatically loaded onto the CZE capillary, providing sample amounts of 0.1-20 pmol. The magnetic mass spectrometer was scanned over the desired mass range, usually between m/z 500 and 2500. Results are shown for separation and analysis of mixtures of synthetic peptides and also for protease digests of recombinant human growth hormone and horse heart cytochrome c.

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