Continuous-flow fast atom bombardment-mass spectrometry of permethylated oligosaccharides: A comparative study of direct mixture analysis with packed capillary column liquid chromatography-fast atom bombardment-mass spectrometry

✍ Scribed by Patrick Boulenguer; Yves Leroy; Josefa Maria Alonso; Jean Montreuil; Guy Ricart; Collin Colbert; Dirk Duquet; Chris Dewaele; Bernard Fournet

• Publisher: Elsevier Science
• Year: 1988
• Tongue: English
• Weight: 431 KB
• Volume: 168
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(88)90024-3

✦ Synopsis

Conventional positive fast atom bombardment (FAB) and continuous-flow FAB analysis were carried out with permethylated lacto-N-tetraose. This latter method, a new approach, has been used to analyze a mixture of permethylated oligosaccharides by liquid chromatography-mass spectrometry (LC-MS) with a packed capillary fused-silica column and the continuous-flow probe as interface. Under these conditions, we found the continuous-flow probe to be superior to the conventional probe because its low matrix level increased the signal-to-noise ratio. The analysis of the mixture of permethylated oligosaccharide alditols obtained from hen ovomucoid by LC-MS using the continuous-flow probe as interface is described.