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The Analysis of Some Corticosteroids and Corticosteroid Esters by Liquid Chromatography Coupled with Frit Fast-atom Bombardment Mass Spectrometry

✍ Scribed by David G. Watson; Alistair G. Davidson; Barry I. Knight

Publisher: John Wiley and Sons
Year: 1997
Tongue: English
Weight: 105 KB
Volume: 11
Category: Article
ISSN: 0951-4198
DOI: 10.1002/(sici)1097-0231(19970228)11:4<415::aid-rcm864>3.0.co;2-2

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✦ Synopsis

A method utilizing a liquid chromatography/mass spectrometry method based on a Frit-fast atom bombardment (FAB) interface was developed for the analysis of corticosteroids and corticosteroid esters. The method used a microbore octadecylsilane column with methanol + water as the mobile phase; 0.5% glycerol was incorporated into the mobile phase as the FAB matrix. Separation of a mixture containing five corticosteroids was achieved. Full-scan spectra were readily obtained for 20 ng of each steroid introduced into the instrument.

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