Convenient, laboratory procedure for producing solid d-arabino-hexos-2-ulose (d-glucosone)

✍ Scribed by Te-ning E. Liu; Beverly Wolf; John Geigert; Saul L. Neidleman; Janice D. Chin; David S. Hirano

• Book ID: 102640731
• Publisher: Elsevier Science
• Year: 1983
• Tongue: English
• Weight: 403 KB
• Volume: 113
• Category: Article
• ISSN: 0008-6215
• DOI: 10.1016/0008-6215(83)88227-5

✦ Synopsis

The production of solid D-avabina-hexos-2-ulose (D-glucosone) from o-glucose by use of an enzyme, pyranose-2-oxidase (EC 1.1.3.10), is described. The enzyme is extracted from the mycelia of Pollpporus obtusus, partially purified, and then immobilized on activated CH-Sepharose 4B. The enzymic conversion of D-glucose into D-glucosone is simple and convenient, and provides a product free from residual D-glucose. Lyophilization of the filtered reaction-solution yields the product, solid D-glucosone. Assay methods have been developed for monitoring the enzymic reaction and evaluating the purity of the final product.