Controlling the kinetics of transgene expression by plasmid design
β Scribed by Nelson S. Yew
- Book ID
- 113417898
- Publisher
- Elsevier Science
- Year
- 2005
- Tongue
- English
- Weight
- 155 KB
- Volume
- 57
- Category
- Article
- ISSN
- 0169-409X
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## Abstract ## Background Efficient delivery and expression of plasmids (pDNA) is a major concern in gene therapy and DNA vaccination using nonβviral vectors. Besides the use of adjuvants, the pDNA vector itself can be designed to maximize survival in nucleaseβrich environments. Homopurineβrich tr
To examine the effects of inflammatory response to plasmid DNA (pDNA) on transgene expression, serum tumor necrosis factor-a (TNF-a) was measured after intravenous injection of pDNA or calf thymus DNA (CT DNA) in the naked or complexed form with cationic liposomes (lipoplex). pDNA with many CpG moti