Contribution to the subunit composition of the 11S globulin from rapeseed (Brassica napus L.)
β Scribed by Raab, B. ;Schwenke, K. D.
- Publisher
- John Wiley and Sons
- Year
- 1986
- Tongue
- English
- Weight
- 120 KB
- Volume
- 30
- Category
- Article
- ISSN
- 0027-769X
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π SIMILAR VOLUMES
The 12 S globulin of rapeseed represents an oligomeric protein with a molecular weight of 300,000. It is composed of 6 subunits, which are arranged in a trigonal. antiprism with the point group symmetry 32 (D3). Each subunit contains smaller units (polypeptide chains) with molecular weights in the
A simplified procedure for the isolation of chromatographic homogeneous albumin and 12 S globulin from rapeseed is proposed. The method includes prepurification of the proteins by means of fractionating precipitation and dissolution with ammonium sulphate and single chromatography on Sephadex G-200.
## Academy of Sciences of the German Democratic Republic A reversible dissociation of the 12 S globulin from rapeseed (Brassica napus L.) depending on ionic strength (Short communication)
## Abstract An isolation procedure for the 12 S rapeseed globulin is described which includes precipitation by dialysis, purification using gel chromatography on Sephadex Gβ200, and ionβexchange chromatography on DEAEβSephadex Aβ50. The isolated globulin represents a neutral protein with an isoele