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Simplified isolation procedure for the 12 S globulin and the albumin fraction from rapeseed (Brassica napus L.)

✍ Scribed by Raab, Barbara ;Schwenke, K. D.

Publisher: John Wiley and Sons
Year: 1984
Tongue: English
Weight: 232 KB
Volume: 28
Category: Article
ISSN: 0027-769X
DOI: 10.1002/food.19840280820

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✦ Synopsis

A simplified procedure for the isolation of chromatographic homogeneous albumin and 12 S globulin from rapeseed is proposed. The method includes prepurification of the proteins by means of fractionating precipitation and dissolution with ammonium sulphate and single chromatography on Sephadex G-200.

The most important advantages of the proposed technique are high yields of purified proteins and a simplification of isolation procedure by reducing the number of chromatographic runs.

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