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Contribution of blotting techniques to the study of rapeseeds (Brassica napus L.) lipases

✍ Scribed by Hatem Belguith; Houssein Khodjet El Khil; Sami Fattouch; Toufik Jridi; Jeannette Ben Hamida


Publisher
John Wiley and Sons
Year
2001
Tongue
English
Weight
663 KB
Volume
22
Category
Article
ISSN
0173-0835

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The 12 S globulin of rapeseed represents an oligomeric protein with a molecular weight of 300,000. It is composed of 6 subunits, which are arranged in a trigonal. antiprism with the point group symmetry 32 (D3). Each subunit contains smaller units (polypeptide chains) with molecular weights in the

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Seven rapeseed varieties were characterized by investigation of the patterns of buffer soluble proteins using polyacrylamide gel electrophoresis and high-performance liquid chromatography (size-exclusion and cation-exchange chromatography). Most pronounced differences were found in proteins not belo