A continuous spectrophotometric assay for the determination of the initial rate of the phosphorylase kinase catalyzed reaction at pH 7.0 is presented. The assay incorporates two coupling enzyme systems: (a) recombinant rabbit skeletal muscle type 1 protein phosphatase catalytic subunit which dephosp
Continuous spectrophotometric assay for glycogen phosphorylase activity
β Scribed by Mar Company; Javier Zuluaga; Juan S. Jimenez
- Book ID
- 119070231
- Publisher
- Elsevier Science
- Year
- 1988
- Tongue
- English
- Weight
- 362 KB
- Volume
- 10
- Category
- Article
- ISSN
- 0141-8130
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π SIMILAR VOLUMES
The physiological role of glycogen phosphorylase is the degradation of glycogen, even though the concentration ratio of glucose-l-P/Pi at equilibrium is ca. 0.28 (equation a) (1) : (glycogen), + Pi = glucose-l-P + (glycogen),-l (a)
An assay for phosphorylase a or b is described in which the rate of release of phosphate from glucose l-phosphate is followed by means of an "instantaneous" method for determination of phosphate. Glycogen phosphorylase is commonly assayed either in the direction of glycogen breakdown using a comple