An automated assay for glycogen phosphorylase
โ Scribed by Richard H. Haschke; Ludwig M.G. Heilmeyer Jr.
- Publisher
- Elsevier Science
- Year
- 1972
- Tongue
- English
- Weight
- 305 KB
- Volume
- 47
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
โฆ Synopsis
The physiological role of glycogen phosphorylase is the degradation of glycogen, even though the concentration ratio of glucose-l-P/Pi at equilibrium is ca. 0.28 (equation a) (1) :
(glycogen), + Pi = glucose-l-P + (glycogen),-l (a)
๐ SIMILAR VOLUMES
An assay for phosphorylase a or b is described in which the rate of release of phosphate from glucose l-phosphate is followed by means of an "instantaneous" method for determination of phosphate. Glycogen phosphorylase is commonly assayed either in the direction of glycogen breakdown using a comple
Glycogen phosphorylase (a-l,4-glucan:orthophosphate glueosyltransferase, EC 2.4.1.1) may be assayed in either the direction of glycogen synthesis or the direction of glycogen phosphorolysis. In the forward direction, phosphorolysis, progress of the reaction can be monitored continuously or as a fixe
As an alternative to rapid filtration washing the glycogen free of any unreacted UDP-["Clglucose by ascending chromatography (ethanol:water, 2: 1) can be used. This technique also makes the filter paper assay of glycogen synthase much faster: The samples are ready for liquid scintillation counting i