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An automated assay for phosphorylase kinase

โœ Scribed by H.P. Jennissen; L.M.G. Heilmeyer Jr.

Publisher
Elsevier Science
Year
1974
Tongue
English
Weight
414 KB
Volume
57
Category
Article
ISSN
0003-2697

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๐Ÿ“œ SIMILAR VOLUMES

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The physiological role of glycogen phosphorylase is the degradation of glycogen, even though the concentration ratio of glucose-l-P/Pi at equilibrium is ca. 0.28 (equation a) (1) : (glycogen), + Pi = glucose-l-P + (glycogen),-l (a)

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An assay for phosphorylase a or b is described in which the rate of release of phosphate from glucose l-phosphate is followed by means of an "instantaneous" method for determination of phosphate. Glycogen phosphorylase is commonly assayed either in the direction of glycogen breakdown using a comple