✦ LIBER ✦

An improved rapid assay of glycogen synthase

✍ Scribed by Emma Villa-Moruzzi; Teresa Locci-Cubeddu; Ettore Bergamini

Publisher: Elsevier Science
Year: 1979
Tongue: English
Weight: 138 KB
Volume: 100
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(79)90243-4

No coin nor oath required. For personal study only.

✦ Synopsis

As an alternative to rapid filtration washing the glycogen free of any unreacted UDP-["Clglucose by ascending chromatography (ethanol:water, 2: 1) can be used. This technique also makes the filter paper assay of glycogen synthase much faster: The samples are ready for liquid scintillation counting in 30 min. Among the other advantages offered by this procedure, we should also mention that blanks are very low, large volumes of ethanol can be saved, and the unreacted UDP-[Wlglucose can be recovered by elution and recycled (it migrates with the front of the solvent).

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