An assay for phosphorylase a or b is described in which the rate of release of phosphate from glucose l-phosphate is followed by means of an "instantaneous" method for determination of phosphate. Glycogen phosphorylase is commonly assayed either in the direction of glycogen breakdown using a comple
A titrimetric assay for glycogen phosphorylase
โ Scribed by K. Palter; A. Lukton
- Publisher
- Elsevier Science
- Year
- 1973
- Tongue
- English
- Weight
- 610 KB
- Volume
- 53
- Category
- Article
- ISSN
- 0003-2697
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๐ SIMILAR VOLUMES
The physiological role of glycogen phosphorylase is the degradation of glycogen, even though the concentration ratio of glucose-l-P/Pi at equilibrium is ca. 0.28 (equation a) (1) : (glycogen), + Pi = glucose-l-P + (glycogen),-l (a)
Glycogen phosphorylase (a-l,4-glucan:orthophosphate glueosyltransferase, EC 2.4.1.1) may be assayed in either the direction of glycogen synthesis or the direction of glycogen phosphorolysis. In the forward direction, phosphorolysis, progress of the reaction can be monitored continuously or as a fixe
In the preceding paper (1) we report the development of a new rapid method for the assay of glycogen phosphorylase. During the development of this method a number of conditions were observed to influence the measurement of enzyme activity in the crude extracts. These have been studied systematicall