Continuous micropreparative trapping in carrier ampholyte-free isoelectric focusing
✍ Scribed by Mirko Deml; Jan Pospíchal; Josef Chmelík
- Book ID
- 103151694
- Publisher
- Elsevier Science
- Year
- 1995
- Tongue
- English
- Weight
- 689 KB
- Volume
- 709
- Category
- Article
- ISSN
- 1873-3778
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📜 SIMILAR VOLUMES
Three proteins (myoglobin, ribonuclease, and cytochrome C) were concentrated, separated, and isolated from a dilute mixture by electrically controlled focusing in carrier-ampholyte-free solution. Electrolytes were modified at both sides of the capillary. The described method is suitable for the micr
## Abstract A simple method of generating pH gradients for use in capillary isoelectric focusing was described and evaluated experimentally. In the proposed scheme, the cone shaped capillary filled with a conventional buffer is used to produce an effect of nonuniform heating which then generate tem