Ampholyte-free isoelectric focusing of proteins in cone shaped capillaries

✍ Scribed by Janusz Pawliszyn; Jiaqi Wu

• Publisher: John Wiley and Sons
• Year: 1993
• Tongue: English
• Weight: 349 KB
• Volume: 5
• Category: Article
• ISSN: 1040-7685
• DOI: 10.1002/mcs.1220050502

✦ Synopsis

Abstract

A simple method of generating pH gradients for use in capillary isoelectric focusing was described and evaluated experimentally. In the proposed scheme, the cone shaped capillary filled with a conventional buffer is used to produce an effect of nonuniform heating which then generate temperature and corresponding pH gradients along the capillary axis. Concentration and separation of proteins by applying a simple isoelectric focusing system based on this principle is demonstrated. An inexpensive absorption imaging detection system is used to continuously monitor progress of the separation and concentration processes.