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Laser-induced fluorescence detection of non-covalently labeled protein in capillary isoelectric focusing

✍ Scribed by Newman S.-K. Sze; Tiemin Huang; Janusz Pawliszyn


Publisher
John Wiley and Sons
Year
2002
Tongue
English
Weight
111 KB
Volume
25
Category
Article
ISSN
1615-9306

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Aequorea victoria green fluorescent protein was assayed by capillary electrophoresis using post-capillary laserinduced fluorescence detection in a sheath flow cuvette. The limit of detection was 3.0 Ο« 10 Οͺ 12 M protein in an injection volume of 17 nL, corresponding to a mass of 3100 molecules.