Continuous kinetic method for the quantitative resolution of structural isomers of arginine and ornithine

✍ Scribed by E. Ballesteros; M. Gallego; M. Valcárcel; F. Grases

• Book ID: 102983780
• Publisher: Elsevier Science
• Year: 1995
• Tongue: English
• Weight: 583 KB
• Volume: 315
• Category: Article
• ISSN: 0003-2670
• DOI: 10.1016/0003-2670(95)00301-f

✦ Synopsis

A flow injection method for the determination of L-arginine and L-omithine based on the inhibition of L-histidine crystallization was developed. The open-closed system used permits turbidimetric multidetection of the signal in the crystallization of L-histidine in the presence of an organic solvent (Zpropanol). The proposed method permits the selective determination of L-arginine (0.3-12 mg 1-l) and L-omithine (0.5-20 mg 1-l) in the presence of their D-enantiomers and other L-amino acids without the need for a prior separation. The relative standard deviation was ca. 2.3% (n = 11). Both amino acids were determined satisfactorily in pharmaceutical preparations, and their recovery after addition to samples was 100 f 3%. The sampling frequency was 7 f 1 h-l.