✦ LIBER ✦

Continuous flow infrared matrix-assisted laser desorption/ionization with a solvent matrix

✍ Scribed by Steven J. Lawson; Kermit K. Murray

Publisher: John Wiley and Sons
Year: 2000
Tongue: English
Weight: 90 KB
Volume: 14
Category: Article
ISSN: 0951-4198
DOI: 10.1002/(sici)1097-0231(20000215)14:3<129::aid-rcm849>3.0.co;2-p

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✦ Synopsis

Continuous flow matrix-assisted laser desorption/ionization (MALDI) was demonstrated with infrared laser desorption and an ethanol matrix. A capillary was used to deliver an analyte solution dissolved in ethanol to a metal frit embedded in a sample stage. Typical flow rates were 1.7&mgrL/min. An optical parametric oscillator tuned to 2.8&mgrm was used for desorption and ionization, and mass analysis was achieved with a 1 m linear time-of-flight mass spectrometer. Flow injection studies were performed with low picomolar quantities of insulin and myoglobin in solutions containing 0.1 to 1.0% glycerol in ethanol. Copyright 2000 John Wiley & Sons, Ltd.

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