✦ LIBER ✦

A New Matrix for Matrix-assisted Laser Desorption/Ionization on Magnetic Sector Instruments with Point Detectors

✍ Scribed by V. S. Kumar Kolli; Ron Orlando

Publisher: John Wiley and Sons
Year: 1996
Tongue: English
Weight: 406 KB
Volume: 10
Category: Article
ISSN: 0951-4198
DOI: 10.1002/(sici)1097-0231(19960610)10:8<923::aid-rcm610>3.0.co;2-e

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✦ Synopsis

A new matrix consisting of a binary mixture of a-cyano-4-hydroxyannamic acid and 5amino-quinoline has been deveioped specifically for matrix-assisted laser desorption/ionization (MALDI) analysis on magnetic sector mass spectrometers with point detectors. The major benefit of this matrix is that it is a viscous liquid with a self-healing surface. These properties provide very long-lasting and reasonably constant ion currents from pmol quantities of analyte, allowing MALDI-magnetic sector spectra to be obtained even though a non-integrating point detector is used. The applicability of this matrix for biological samples is demonstrated by analyzing a mixture of complex N-linked oligosaccharides with the new liquid matrix and with several traditional solid MALDI matrices both on magnetic sector and time-of-flight instruments.

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