𝔖 Bobbio Scriptorium
✦   LIBER   ✦

A new procedure for quantitative correction of matrix-assisted laser desorption/ionization time-of-flight mass spectrometric response

✍ Scribed by Emilio Scamporrino; Pietro Maravigna; Daniele Vitalini; Placido Mineo

Publisher
John Wiley and Sons
Year
1998
Tongue
English
Weight
114 KB
Volume
12
Category
Article
ISSN
0951-4198

No coin nor oath required. For personal study only.

✦ Synopsis

A simple procedure for the correction of the decreasing detection response in a Matrix-assisted laser desorption/Ionization time-of-flight (MALDI-TOF) mass spectrum with increasing ion mass is reported. On the basis of the experimental MALDI-TOF mass spectrum of a mixture of four polymethylmethacrylate (PMMA) standards (molecular masses in the range 4-100 kDa), present in equal molar abundance, an equation describing the MALDI-TOF response has been derived. This equation has been used to correct any subsequently recorded MALDI spectrum. As an example, the procedure has been applied here to normalize the MALDI-TOF mass spectrum of a 1,20-di(bisphenoxy-A)eicosane based copolyformal (I) containing porphyrin units in the main chain.

πŸ“œ SIMILAR VOLUMES

Matrix-assisted laser desorption/ionizat
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometric analysis of some conducting polymers
✍ Folch, Inma; BorrοΏ½s, Salvador; Amabilino, David B.; Veciana, Jaume πŸ“‚ Article πŸ“… 2000 πŸ› John Wiley and Sons 🌐 English βš– 94 KB πŸ‘ 2 views

The application of laser desorption/ionization and matrix-assisted laser desorption/ionization mass spectrometry to the study of the structure of some conducting polymers was investigated. A methodology was developed and the experimental conditions for the characterization of these polymers were est

Quantitative analysis of antibiotics by
Quantitative analysis of antibiotics by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
✍ Yong-Chien Ling; Lihnian Lin; Yi-Ting Chen πŸ“‚ Article πŸ“… 1998 πŸ› John Wiley and Sons 🌐 English βš– 242 KB πŸ‘ 2 views

Comparative studies of the matrix-assisted laser desorption/ionization (MALDI) of 30 antibiotics were made using a-cyano-4-hydroxycinnamic acid (HCCA), 2,5-dihydroxybenzoic acid (DHB), 5,10,15,20tetrakis(4-hydroxyphenyl)-21H,23H-porphyrin and meso-tetra(N-methyl-4-pyridyl)porphyrin matrices. Most an

Ionization mechanism of oligonucleotides
Ionization mechanism of oligonucleotides in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
✍ Yufei Kong; Yifei Zhu; Jing-Yuan Zhang πŸ“‚ Article πŸ“… 2000 πŸ› John Wiley and Sons 🌐 English βš– 171 KB πŸ‘ 2 views

The ionization of nucleosides in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry was systematically investigated using adenine (A), thymine (T), guanine (G) and cytosine (C) with several common matrices. Experimental results of the protonation and deprotonation of the ba

Electrospray sample preparation for impr
Electrospray sample preparation for improved quantitation in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
✍ Russell R. Hensel; Richard C. King; Kevin G. Owens πŸ“‚ Article πŸ“… 1997 πŸ› John Wiley and Sons 🌐 English βš– 185 KB πŸ‘ 2 views

The goal of this work is the development of a rapid and objective matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) method for the quantitation of peptides and proteins in human plasma suitable for use in the Good Laboratory Practices (GLP) environment, where

Precalibration of matrix-assisted laser
Precalibration of matrix-assisted laser desorption/ionization-time of flight spectra for peptide mass fingerprinting
✍ Assaf Wool; Zeev Smilansky πŸ“‚ Article πŸ“… 2002 πŸ› John Wiley and Sons 🌐 English βš– 107 KB πŸ‘ 1 views

Identification of proteins using matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) peptide mass fingerprinting (PMF) is a key technique in proteomics. The method is known to be sensitive as well as amenable to high-throughput operation, but the resulting identifications suffer f