𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Continuous Culture of a B-Immunocyte from Rheumatoid Synovium

✍ Scribed by Denys K. Ford; J. Donald Smiley

Book ID
102750893
Publisher
John Wiley and Sons
Year
1973
Tongue
English
Weight
523 KB
Volume
16
Category
Article
ISSN
0004-3591

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✦ Synopsis

Abstract

A lymphocyte cell line was established from the culture of rheumatoid synovium and the cell strain continues to proliferate after more than a year in the culture. The exposure of the cells to ^14^C‐amino acids has demonstrated that they manufacture IgG and IgM and, thus, they can be regarded as B‐immunocytes.

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The presence of the lysosomal proteinases cathepsin B and cathepsin D at extracellular sites in rheumatoid synovium was demonstrated using the antibody capture technique. Unlike cathepsin D, the cysteine proteinase cathepsin B was commonly detected only at the edges of the synovial explants. Radioim