Packed bed hollow fiber membrane reactors were used to carry out organic phase biocatalysis at constant water activity. The performance of the device was tested by carrying out the esterification of dodecanol and decanoic acid in hexane. Lipase from Candida rugosa, immobilized on microporous polypro
Continuous control of water activity during biocatalysis in organic media
β Scribed by Ernst Wehtje; Ingemar Svensson; Patrick Adlercreutz; Bo Mattiasson
- Publisher
- Springer-Verlag
- Year
- 1993
- Tongue
- English
- Weight
- 501 KB
- Volume
- 7
- Category
- Article
- ISSN
- 0951-208X
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β¦ Synopsis
This paper describes a newly developed technique to adjust and control the water activity in enzymatic reactions in organic media. A saturated salt solution of known water activity is circulated inside a silicone tube, submerged into the reaction medium. The circulating solution can both absorb and release water. Water activity control during lipase catalyzed esterification was demonstrated with diisopropyl ether as solvent.
π SIMILAR VOLUMES
## Abstract A water activity control system for enzymatic synthesis in organic media, for litreβscale reactors has been constructed. Water activity, __a__~w~, is a key factor when using enzymes in nonβconventional media and the optimum value varies for different enzymes. The control system consists
The V,, and KM of various forms of lipase from Pseudomonas cepacia ( powder, adsorbed onto Celite or covalently linked to polyethylene glycol ) were determined in organic solvents preequilibrated to water activities ( a, ) from ~0.1 to 0.84. The model reaction was the transesterification between n-o