Significant changes of nuclear chromatin in the Capan-2 cells were observed under fluorescent microscopy after LED-activated Pa. In the control groups, the nuclear chromatin was stained a less bright blue. After being irradiated by LED-activated Pa, the blue emission light in the Capan-2 cells was m
Contents: Laser Phys. Lett. 6/2011
- Publisher
- John Wiley and Sons
- Year
- 2011
- Tongue
- English
- Weight
- 253 KB
- Volume
- 8
- Category
- Article
- ISSN
- 1612-2011
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โฆ Synopsis
In order to analyze the effect of milling parameters on the sub-ฮผm grating structures, the technique for creating cross-sections of the milled structures was optimized. The cross-sectioning was done in situ using the same FIB machine that produced the grating structure. The optimized method started with local FIB-induced deposition of a Pt layer on top of the region of interest in order to prevent redeposition when milling the crosssection and to provide a contrast for scanning electron microscopy (SEM) imaging. The next step involved milling a large-area hole with a sloped angle to avoid long milling times. Finally, a line-by-line scan (termed cleaning cross-section) was applied at a lower current (typically 28 pA) to facilitate a high contrast image, as depicted in Cover Picture. Analysis of the crosssectional profile of the grating structures was performed by use of SEM available in the same machine. (Cover picture:
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