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Contacts between the lac Repressor and Thymines in the lac Operator

✍ Scribed by Ronald Ogata and Walter Gilbert

Book ID
123654695
Publisher
National Academy of Sciences
Year
1977
Tongue
English
Weight
993 KB
Volume
74
Category
Article
ISSN
0027-8424

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In the lac operon, the existence of a secondary repressor binding site, inside Z gene, had been inferred from in vitro binding studies (Reznikoff et al., 1974; Gilbert et al., 1975). A series of deletions have been constructed from a lac transducing lambda bacteriophage. Some of those deleted bacter

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A model is suggested for the lac repressor binding to the lac operator in which the repressor polypeptide chain sequences from Gly 14 to Ala 32 and from Ala 53 to Leu 71 are involved in specific interaction with operator DNA. A correspondence between the protein and DNA sequences is found which expl