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Binding of lac repressor to the secondary lac operator in Escherichia coli

✍ Scribed by Rambach, Alain ;Lebastard, Mai

Publisher: Springer
Year: 1978
Tongue: English
Weight: 234 KB
Volume: 166
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00285926

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✦ Synopsis

In the lac operon, the existence of a secondary repressor binding site, inside Z gene, had been inferred from in vitro binding studies (Reznikoff et al., 1974; Gilbert et al., 1975). A series of deletions have been constructed from a lac transducing lambda bacteriophage. Some of those deleted bacteriophages have still the property of derepressing a chromosomal lac operon, even though they do not contain any more the lac operator. This phenomenon is an indication that the secondary repressor binding site is also active in vivo.

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