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Construction of two stable bifunctional plasmids for Streptomyces spp. and Escherichia coli

✍ Scribed by Sonja Durajlija; Jasenka Pigac; Vera Gamulin

Book ID
109316935
Publisher
John Wiley and Sons
Year
1991
Tongue
English
Weight
308 KB
Volume
83
Category
Article
ISSN
0378-1097

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πŸ“œ SIMILAR VOLUMES

Construction and characterization of a p
Construction and characterization of a plasmid coding for a fragment of the Escherichia coli recA protein
✍ Little, John W. πŸ“‚ Article πŸ“… 1979 πŸ› Springer 🌐 English βš– 1002 KB

The E. coli recA gene was cloned from the phage lambda precA into the vector pBR313. A plasmid, pJL3, was also isolated by cloning a portion of the recA gene into the vector pBR322. pJL3 coded for a fragment of the recA protein 34 Kd (kilodaltons) in size (compared to 40 Kd for the intact protein).