Construction of an equalized cDNA librar
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Takayuki Kohchi; Kotomi Fujishige; Kanji Ohyama
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Article
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1995
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John Wiley and Sons
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English
โ 342 KB
Using a kinetic approach, a cDNA library composed of almost equal representations of all genes expressed in the aerial parts of 2โweekโold __Arabidopsis__ was constructed. A cDNA was synthesized with an oligo dT primer containing a __Not__l site. A linker containing the nucleotide sequence of __Sse_