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Toward a Whole cDNA Catalog: Construction of an Equalized cDNA Library from Mouse Embryos

โœ Scribed by Naomi Takahashi; Minoru S.H. Ko

Book ID
115612330
Publisher
Elsevier Science
Year
1994
Tongue
English
Weight
843 KB
Volume
23
Category
Article
ISSN
0888-7543

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Using a kinetic approach, a cDNA library composed of almost equal representations of all genes expressed in the aerial parts of 2โ€weekโ€old __Arabidopsis__ was constructed. A cDNA was synthesized with an oligo dT primer containing a __Not__l site. A linker containing the nucleotide sequence of __Sse_