๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

Construction of a mouse blastocyst cDNA library by PCR amplification from total RNA

โœ Scribed by Catherine M. Corrick; Mary J. Silvestro; Mireille H. Lahoud; Greg J. Allen; Martin J. Tymms; Ismail Kola

Book ID
111658622
Publisher
John Wiley and Sons
Year
1996
Tongue
English
Weight
959 KB
Volume
43
Category
Article
ISSN
1040-452X

No coin nor oath required. For personal study only.

๐Ÿ“œ SIMILAR VOLUMES

cDNA library construction from small amo
cDNA library construction from small amounts of unfractionated RNA: Association of cDNA synthesis with polymerase chain reaction amplification
โœ Christine Domec; Bertrand Garbay; Michel Fournier; Jacques Bonnet ๐Ÿ“‚ Article ๐Ÿ“… 1990 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 942 KB

We describe here a general and simple procedure for cDNA library construction making use of in vitro amplification of cDNA by polymerase chain reaction (PCR). The first-strand cDNA is synthesized from total RNA with a primer EcoRI-(dT)17 and oligo(dG) tailed. An oligonucleotide, EcoRI-BamHI-(dC)13,