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Construction of cDNA Libraries from Small Amounts of Total RNA Using the Suppression PCR Effect

โœ Scribed by Konstantin Lukyanov; Luda Diatchenko; Alex Chenchik; Amulia Nanisetti; Paul Siebert; Natalia Usman; Mikhail Matz; Sergey Lukyanov


Book ID
115579640
Publisher
Elsevier Science
Year
1997
Tongue
English
Weight
113 KB
Volume
230
Category
Article
ISSN
0006-291X

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cDNA library construction from small amo
โœ Christine Domec; Bertrand Garbay; Michel Fournier; Jacques Bonnet ๐Ÿ“‚ Article ๐Ÿ“… 1990 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 942 KB

We describe here a general and simple procedure for cDNA library construction making use of in vitro amplification of cDNA by polymerase chain reaction (PCR). The first-strand cDNA is synthesized from total RNA with a primer EcoRI-(dT)17 and oligo(dG) tailed. An oligonucleotide, EcoRI-BamHI-(dC)13,