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Construction of aSaccharomyces cerevisiaestrain able to ferment cellobiose

โœ Scribed by Ana C. Adam; Julio Polaina

Publisher
Springer-Verlag
Year
1991
Tongue
English
Weight
385 KB
Volume
20
Category
Article
ISSN
0172-8083

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โœฆ Synopsis

The bglA gene, encoding a beta-glucosidase from Bacillus polymyxa, has been expressed in Saccharomyces cerevisiae under control of the CYC-GAL promoter inducible by galactose. The expression of bglA-encoded activity in the strain used as a host was not sufficient to allow its growth with cellobiose as a carbon source. However, a recessive mutation in a gene designated cem1 has been obtained which, combined with the expression of beta-glucosidase activity, allows the growth of S. cerevisiae on cellobiose. The expression of the blgA gene in a cem1 strain confers on S. cerevisiae the capability for an efficient fermentation of cellobiose, as detected by the formation of CO2.

๐Ÿ“œ SIMILAR VOLUMES

Adaptation of aSaccharomyces cerevisiaes
Adaptation of aSaccharomyces cerevisiaestrain to high copper concentrations
โœ Ileana Sarais; Marisa Manzano; Marco Bertoldi; Paolo Romandini; Mariano Beltrami ๐Ÿ“‚ Article ๐Ÿ“… 1994 ๐Ÿ› Springer Netherlands ๐ŸŒ English โš– 629 KB

A strain of Saccharomyces cerevisiae has been adapted to increasing concentrations of copper at two different pH values. The growth curve at pH 5.5 is characterized by a time generation increasing with the amount of added copper. A significant decrease of cell volume as compared with the control is