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Construction of an integrative food-grade expression system for Bacillus subtilis

✍ Scribed by Yu Xia; Wei Chen; Xiaoyan Fu; Hao Zhang; Sheng Yang; Xiaolin Ding


Book ID
116487884
Publisher
Elsevier Science
Year
2005
Tongue
English
Weight
184 KB
Volume
38
Category
Article
ISSN
0963-9969

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We demonstrate for the first time, an expression system mimicking serine alkaline protease synthesis and secretion, producing native form of human growth hormone (hGH) from Bacillus subtilis. A hybrid-gene of two DNA fragments, i.e., signal (pre-) DNA sequence of B. licheniformis serine alkaline pro