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Construction of a Marker Rescue System in Bacillus subtilis for Detection of Horizontal Gene Transfer in Food

โœ Scribed by Mitra Kharazmi; Walter P. Hammes; Christian Hertel

Book ID
114493542
Publisher
Elsevier Science
Year
2002
Tongue
German
Weight
106 KB
Volume
25
Category
Article
ISSN
0723-2020

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Novel plasmid marker rescue transformati
Novel plasmid marker rescue transformation system for molecular cloning in Bacillus subtilis enabling direct selection of recombinants
โœ Haima, Peter ;Bron, Sierd ;Venema, Gerard ๐Ÿ“‚ Article ๐Ÿ“… 1990 ๐Ÿ› Springer ๐ŸŒ English โš– 808 KB

A versatile plasmid marker rescue transformation system was developed for homology-facilitated cloning in Bacillus subtilis. It is based on the highly efficient host-vector system 6GM15-pHPS9, which allows the direct selection of recombinants by means of beta-galactosidase alpha-complementation. The