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Construction of a fusion gene comprising the Taka-amylase A promoter and the Escherichia coli β-glucuronidase gene and analysis of its expression in Aspergillus oryzae

✍ Scribed by Tada, Setsuzo ;Gomi, Katsuya ;Kitamoto, Katsuhiko ;Takahashi, Kojiro ;Tamura, Gakuzo ;Hara, Shodo

Publisher
Springer
Year
1991
Tongue
English
Weight
722 KB
Volume
229
Category
Article
ISSN
0026-8925

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✦ Synopsis

Northern blot analysis of glucose-grown and starch-grown mycelia of Aspergillus oryzae RIB40 was conducted using the cloned Taka-amylase A (TAA) gene as a probe. The amount of mRNA homologous to the TAA gene was increased when this fungus was grown with starch as a sole carbon source. In order to analyze the induction mechanism, we inserted the Escherichia coli uidA gene encoding beta-glucuronidase (GUS) down-stream of the TAA promoter and introduced the resultant fusion gene into the A. oryzae genome. Production of a functional GUS protein was induced by starch, but not by glucose. When the effects of various sugars on expression of the fusion gene were examined, the results suggested that the expression of the fusion gene was under control of the TAA gene promoter.

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