Transformation ofTrichoderma virideusing theNeurospora crassa pyr4 gene and its use in the expression of a Taka-amylase A gene fromAspergillus oryzae

Construction of a fusion gene comprising

Construction of a fusion gene comprising the Taka-amylase A promoter and the Escherichia coli β-glucuronidase gene and analysis of its expression in Aspergillus oryzae

✍ Tada, Setsuzo ;Gomi, Katsuya ;Kitamoto, Katsuhiko ;Takahashi, Kojiro ;Tamura, Ga 📂 Article 📅 1991 🏛 Springer 🌐 English ⚖ 722 KB

Northern blot analysis of glucose-grown and starch-grown mycelia of Aspergillus oryzae RIB40 was conducted using the cloned Taka-amylase A (TAA) gene as a probe. The amount of mRNA homologous to the TAA gene was increased when this fungus was grown with starch as a sole carbon source. In order to an