A Neurospora gene bank in plasmid pRK9 was used to complement pyrimidine auxotrophs in E. coli. Two plasmids were obtained that complement a pyrF mutant of E. coli. These plasmids hybridise to Neurospora DNA and transform a pyr-4 strain of Neurospora. The promoter used in expressing the orotidine 5'
Cloning of the ARO cluster gene of Neurospora crassa and its expression in Escherichia coli
β Scribed by Catcheside, D. E. A. ;Storer, P. J. ;Klein, B.
- Publisher
- Springer
- Year
- 1985
- Tongue
- English
- Weight
- 699 KB
- Volume
- 199
- Category
- Article
- ISSN
- 0026-8925
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