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Construction and characterization of a peanutHindIII BAC library

✍ Scribed by B. Yüksel; A. H. Paterson

Publisher
Springer
Year
2005
Tongue
English
Weight
337 KB
Volume
111
Category
Article
ISSN
0040-5752

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A bovine genomic large‐insert bacterial artificial chromosome (BAC) library has been constructed from leukocytes of a Holstein‐Friesian male. Size fractionated __Dpn__II‐digested genomic DNA was ligated to the dephosphorylated __Bam__H1 ends of a pBACe3·6 vector. Approximately 8·3×104 individual BAC