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Construction of a BAC library of Korean ginseng and initial analysis of BAC-end sequences

✍ Scribed by C. P. Hong; S. J. Lee; J. Y. Park; P. Plaha; Y. S. Park; Y. K. Lee; J. E. Choi; K. Y. Kim; J. H. Lee; J. Lee; H. Jin; S. R. Choi; Y. P. Lim


Publisher
Springer
Year
2004
Tongue
English
Weight
286 KB
Volume
271
Category
Article
ISSN
1617-4615

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A bovine genomic large‐insert bacterial artificial chromosome (BAC) library has been constructed from leukocytes of a Holstein‐Friesian male. Size fractionated __Dpn__II‐digested genomic DNA was ligated to the dephosphorylated __Bam__H1 ends of a pBACe3Β·6 vector. Approximately 8Β·3Γ—104 individual BAC