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Construction and characterization of a BAC-library for a key pollinator, the bumblebeeBombus terrestrisL.

✍ Scribed by L. Wilfert; M. Muñoz Torres; C. Reber-Funk; R. Schmid-Hempel; J. Tomkins; J. Gadau; P. Schmid-Hempel


Publisher
Springer
Year
2008
Tongue
English
Weight
254 KB
Volume
56
Category
Article
ISSN
0020-1812

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Construction and characterization of a g
✍ J Buitkamp; S Kollers; G Durstewitz; R Fries; K Welzel; K Schäfer; A Kellermann; 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 137 KB

A bovine genomic large‐insert bacterial artificial chromosome (BAC) library has been constructed from leukocytes of a Holstein‐Friesian male. Size fractionated __Dpn__II‐digested genomic DNA was ligated to the dephosphorylated __Bam__H1 ends of a pBACe3·6 vector. Approximately 8·3×104 individual BAC