Five mouse-human hybridomas, H2, H3, V1, V2 and V6 cells secreting anti-virus human monoclonal immunoglobulin G (IgG) were cultured in serum-free media at high density in a settling perfusion culture vessel with an inner cell sedimentation zone. The H2, H3 and V6 cells reached a density of 107 cells
Constitutive expression of human angiostatin inPichia pastorisby high-density cell culture
โ Scribed by A. L. Zhang; T. Y. Zhang; J. X. Luo; S. C. Chen; W. J. Guan; C. Y. Fu; S. Q. Peng; H. L. Li
- Publisher
- Springer-Verlag
- Year
- 2006
- Tongue
- English
- Weight
- 363 KB
- Volume
- 34
- Category
- Article
- ISSN
- 1476-5535
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Mouse-human hybridoma X87X ceils were cultivated using a novel perfusion culture apparatus provided with three-settling zones to separate the cells from the culture medium by gravitational settling. The maximum viable cell density in a serum-free culture medium attained 3.0 x 10 v cells/ml, when the