Conjugative chromosomal gene transfer inBacillus subtilis
β Scribed by O. V. Lotareva; V. Z. Nezametdinova; A. A. Prozorov
- Book ID
- 110199372
- Publisher
- SP MAIK Nauka/Interperiodica
- Year
- 2007
- Tongue
- English
- Weight
- 220 KB
- Volume
- 43
- Category
- Article
- ISSN
- 1022-7954
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
The native promoter of a xylanase gene isolated from Closlriditrm therntocelhrm was replaced with a strong promoter screened from Bacillus strbtilis chromosomes. A part of the C-terminal region of the gene which is not related to the xylanase activity was removed. With the modified xylanase gene, B.
A gene for aUosteric lactate dehydrogenase (LDH) of Lactobacillus casei ATCC393 was transferred into Bacillus subtHis. The LDH was produced in a growth-associated type, and comprised up to 40 % of the total cellular protein. The maximum specific activity in the transformant was 208 U/mg protein whic
The conjugative enterococcal transposons Tn916 and Tn919 were introduced into Bacillus megaterium by a filtermating technique. The transfer frequencies obtained ranged from 1.3Γ10(-6) to 6.6Γ10(-7). The transposons integrated stably into the B. megaterium chromosome. Tn916 could generate auxotrophs