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Expression of theLactobacillus caseilactate dehydrogenase gene inBacillus subtilis

โœ Scribed by Sungmin F. Kim; Seung J. Baek; M. Y. Pack

Book ID
104633161
Publisher
Springer Netherlands
Year
1994
Tongue
English
Weight
221 KB
Volume
16
Category
Article
ISSN
0141-5492

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โœฆ Synopsis

A gene for aUosteric lactate dehydrogenase (LDH) of Lactobacillus casei ATCC393 was transferred into Bacillus subtHis. The LDH was produced in a growth-associated type, and comprised up to 40 % of the total cellular protein. The maximum specific activity in the transformant was 208 U/mg protein which was approximately 16 times higher than in L. casei or in the previously constructed Escherichia coil transformant.

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