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Conjugal transfer of enterococcal transposons inBacillus megaterium

✍ Scribed by M. E. Floccari; B. S. Méndez

Book ID
104723840
Publisher
Springer
Year
1994
Tongue
English
Weight
719 KB
Volume
10
Category
Article
ISSN
1573-0972

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✦ Synopsis

The conjugative enterococcal transposons Tn916 and Tn919 were introduced into Bacillus megaterium by a filtermating technique. The transfer frequencies obtained ranged from 1.3×10(-6) to 6.6×10(-7). The transposons integrated stably into the B. megaterium chromosome. Tn916 could generate auxotrophs and was transferred from B. megaterium Tn916 transconjugants to other species.

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The conjugative transposon Tn925: enhanc
The conjugative transposon Tn925: enhancement of conjugal transfer by tetracycline in Enterococcus faecalis and mobilization of chromosomal genes in Bacillus subtilis and E. faecalis
✍ Torres, Olga R. ;Korman, Ruth Z. ;Zahler, Stanley A. ;Dunny, Gary M. 📂 Article 📅 1991 🏛 Springer 🌐 English ⚖ 776 KB

The effects of tetracycline on transfer of the conjugative, tetracycline-resistance transposon. Tn925, as well as the ability of the transposon to promote the transfer of chromosomal genes was examined in Enterococcus faecalis and Bacillus subtilis. To test for chromosomal transfer, multiply-marked