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Conformational Perturbation of the Envelope Glycoprotein gp120 of Human Immunodeficiency Virus Type 1 by Soluble CD4 and the Lectin Succinyl Con A

✍ Scribed by Ranajit Pal; Anthony DeVico; Stephen Rittenhouse; M.G. Sarngadharan


Book ID
112269194
Publisher
Elsevier Science
Year
1993
Tongue
English
Weight
325 KB
Volume
194
Category
Article
ISSN
0042-6822

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Lateral diffusion of CD4 on the surface
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The envelope glycoprotein (gpl20) of HIV-1 was labeled with fluorescein by using 6- [4,6-dichlorotriazinyl]aminofluorescein. The labeled glycoprotein was found to bind to CD4-positive CEM cells. Monoclonal antibody OKT4a but not OKT4 blocked this binding. Similar specific binding of fluorescein-labe