Computer construction of isokinetic sucrose centrifugation gradients
β Scribed by Robin T. Vollmer
- Publisher
- Elsevier Science
- Year
- 1974
- Tongue
- English
- Weight
- 558 KB
- Volume
- 7
- Category
- Article
- ISSN
- 0010-4809
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β¦ Synopsis
The mathematical framework of isokinetic sucrose density gradients for the ultracentrifuge is discussed, and based on this development a highly automated computer program to calculate those gradients is presented. The program utilizes the iterative chord method and Marquardt's NLIN program to respectively calculate and fit the isokinetic gradient to a gradient making apparatus function.
π SIMILAR VOLUMES
Computer calculated isokinetic gradients provide superior uniform resolution for zone centrifugation of macromolecules and particles of known density (l-3). The technique is particularly useful in that it has the capacity to provide sedimentation coefficients without cumbersome routine computations
The presence of a relatively small number of red cells was found to affect DNA sedimentation profile of normal lymphocytes and acute leukemia cells, as observed by the alkaline sucrose gradient centrifugation technique coupled with the fluorometric measurement of DNA. Significant alteration was obse