The wild-type trp repressor of E. coli bound 5-methoxytryptophan, a Trp analogue, less tightly than Trp. A mutant repressor (Val-+Ala) that should bind 5-methoxytryptophan preferentially to Trp was computationally designed by free-energy calculations accompanied by free-energy decomposition. The des
✦ LIBER ✦
Computational design, construction, and characterization of a set of specificity determining residues in protein–protein interactions
✍ Scribed by Chioko Nagao; Nozomi Izako; Shinji Soga; Samia Haseeb Khan; Shigeki Kawabata; Hiroki Shirai; Kenji Mizuguchi
- Book ID
- 112179726
- Publisher
- John Wiley and Sons
- Year
- 2012
- Tongue
- English
- Weight
- 847 KB
- Volume
- 80
- Category
- Article
- ISSN
- 0887-3585
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