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Complexation of Proteins with a Strong Polyanion in an Aqueous Salt-free System

✍ Scribed by Tsuboi, Atsushi; Izumi, Tsuyoshi; Hirata, Mitsuo; Xia, Jiulin; Dubin, Paul L.; Kokufuta, Etsuo


Book ID
111899520
Publisher
American Chemical Society
Year
1996
Tongue
English
Weight
504 KB
Volume
12
Category
Article
ISSN
0743-7463

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An aqueous two-phase system containing 7% Dextran T 500-5% polyethylene glycol (PEG) 6000 has been adopted for rapid selective stepwise extractions of high-mobility-group proteins and histones from both isolated chromatin and intact nuclei of calf thymus. After the dissociated proteins in the PEG ph