Effects of Surface Charge Distribution of Proteins in Their Complexation with Polyelectrolytes in an Aqueous Salt-Free System
β Scribed by Takahashi, Daisuke; Kubota, Yuichi; Kokai, Kenji; Izumi, Tsuyoshi; Hirata, Mitsuo; Kokufuta, Etsuo
- Book ID
- 120301549
- Publisher
- American Chemical Society
- Year
- 2000
- Tongue
- English
- Weight
- 263 KB
- Volume
- 16
- Category
- Article
- ISSN
- 0743-7463
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π SIMILAR VOLUMES
## Abstract Correlations to describe the effect of surface hydrophobicity and charge of proteins with their partition coefficient in aqueous twoβphase systems were investigated. Polyethylene glycol (PEG) 4000/phosphate, sulfate, citrate, and dextran systems in the presence of low (0.6% w/w) and hig
A series of charge-modified thaumatins with different values of surface charge were partitioned in aqueous twophase systems (ATPS) to study the effect of surface charge as a single property on partitioning. Electrophoretic mobility of the proteins in titration curves was used as a measure of surface